Brazilian ground beef authentication by multiplex polymerase chain reaction

• Main Authors: Andrey Carlos do Sacramento de Oliveira, Silvia Cristina da Silva Pedroso, Diogo José Cardilli, Fábio Pereira Leivas Leite, Gabrielle Virgínia Lopes Ferreira, Andréia Silva da Silva, Talita Bandeira Roos, Carina Martins de Moraes, Roberta Sales Sousa, Roseli do Socorro Dias Monteiro
• Format: Article
• Language: English
• Published: Universidade Federal de Santa Maria 2018-02-01
• Series: Ciência Rural
• Subjects: identificação de espécies; Bos taurus; Bubalus bubalis; DNA; adulteração
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000200652&lng=en&tlng=en
• ISSN: 1678-4596

ABSTRACT: The aim of the present study was to assess the efficacy ofmultiplex PCR in detecting the adulterationof commercially available ground beefvia addition and/orsubstitution ofground buffalo meat. Experimentally adulterated ground beefsamples were prepared in triplicate, and dilutions of DNA from Bos taurus and Bubalusbubalis were prepared to determine the detection limit of the method. Concurrently, 91 ground meatsamples sold as “ground beef” were collected from differentstores in northern Brazil andanalyzed bymultiplex PCR. Buffalo DNA was detected in 17.5% of the collected ground meat samples.Our results showed that multiplex PCR is an efficient method for detectingthe incorporation of groundbuffalo meatatpercentages ranging from 10 to 100% and the incorporation of beef at percentages ranging from0.1 to 100% intoground meat samples.