Unveiling the K+-sensitivity of cell metabolism using genetically encoded, FRET-based K+, glucose, and ATP biosensors

Unveiling the K+-sensitivity of cell metabolism using genetically encoded, FRET-based K+, glucose, and ATP biosensors

Summary: Investigating dynamic changes of mitochondrial ATP and cytosolic glucose levels of single living cells over time by genetically encoded biosensors provides an informative readout of their metabolic activities. Here, we describe how to monitor the metabolic K+-sensitivity of HEK293 cells exp...

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Bibliographic Details
Main Authors: Helmut Bischof, Sandra Burgstaller, Wolfgang F. Graier, Robert Lukowski, Roland Malli
Format: Article
Language:English
Published: Elsevier 2021-12-01
Series:STAR Protocols
Subjects:
Cancer
Cell Biology
Cell culture
Chemistry
Metabolism
Microscopy
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721005499

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http://www.sciencedirect.com/science/article/pii/S2666166721005499

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