Manufacturing NKG2D CAR-T cells with piggyBac transposon vectors and K562 artificial antigen-presenting cells

Manufacturing NKG2D CAR-T cells with piggyBac transposon vectors and K562 artificial antigen-presenting cells

Non-viral platforms can be applied rapidly and cost-effectively for chimeric antigen receptor (CAR)-T cell manufacturing. In the present paper, we describe in detail a clinically relevant manufacturing process for NKG2D CAR-T cells through electroporation of CAR-encoding piggyBac transposon plasmids...

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Bibliographic Details
Main Authors: Johan C.K. Tay, Junjian Wang, Zhicheng Du, Yu Yang Ng, Zhendong Li, Yuefang Ren, Chang Zhang, Jianqing Zhu, Xue Hu Xu, Shu Wang
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:Molecular Therapy: Methods & Clinical Development
Subjects:
piggyBac transposon
K562 aAPCs
NKG2D CAR-T cells
Online Access:http://www.sciencedirect.com/science/article/pii/S2329050121000383

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http://www.sciencedirect.com/science/article/pii/S2329050121000383

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