Imaging and Analysis of Presynaptic Calcium Influx in Cultured Neurons Using synGCaMP6f

Imaging and Analysis of Presynaptic Calcium Influx in Cultured Neurons Using synGCaMP6f

Presynaptic Ca2+ influx through voltage-gated calcium channels (VGCCs) is a key step in synaptic transmission that links action potential (AP)-derived depolarization to vesicle release. However, investigation of presynaptic Ca2+ influx by patch clamp recordings is difficult due to the small size of...

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Bibliographic Details
Main Authors: Johannes Brockhaus, Bianca Brüggen, Markus Missler
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-04-01
Series:Frontiers in Synaptic Neuroscience
Subjects:
synapse
live imaging
paired pulse facilitation
high voltage activated calcium channels
synaptic transmission
genetically encoded calcium indicator
Online Access:https://www.frontiersin.org/article/10.3389/fnsyn.2019.00012/full

Internet

https://www.frontiersin.org/article/10.3389/fnsyn.2019.00012/full

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