A New Generation of FRET Sensors for Robust Measurement of Gαi1, Gαi2 and Gαi3 Activation Kinetics in Single Cells.

A New Generation of FRET Sensors for Robust Measurement of Gαi1, Gαi2 and Gαi3 Activation Kinetics in Single Cells.

G-protein coupled receptors (GPCRs) can activate a heterotrimeric G-protein complex with subsecond kinetics. Genetically encoded biosensors based on Förster resonance energy transfer (FRET) are ideally suited for the study of such fast signaling events in single living cells. Here we report on the c...

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Bibliographic Details
Main Authors: Jakobus van Unen, Anette D Stumpf, Benedikt Schmid, Nathalie R Reinhard, Peter L Hordijk, Carsten Hoffmann, Theodorus W J Gadella, Joachim Goedhart
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2016-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4723041?pdf=render

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http://europepmc.org/articles/PMC4723041?pdf=render

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