Activity of the Human Rhinovirus 3C Protease Studied in Various Buffers, Additives and Detergents Solutions for Recombinant Protein Production.

Proteases are widely used to remove affinity and solubility tags from recombinant proteins to avoid potential interference of these tags with the structure and function of the fusion partner. In recent years, great interest has been seen in use of the human rhinovirus 3C protease owing to its string...

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Bibliographic Details
Main Authors: Raheem Ullah, Majid Ali Shah, Soban Tufail, Fouzia Ismat, Muhammad Imran, Mazhar Iqbal, Osman Mirza, Moazur Rhaman
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2016-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4836831?pdf=render