Efficient genetic transformation of Lotus corniculatus L. using a direct shoot regeneration protocol, stepwise hygromycin B selection, and a super-binary Agrobacterium tumefaciens vector

Efficient genetic transformation of Lotus corniculatus L. using a direct shoot regeneration protocol, stepwise hygromycin B selection, and a super-binary Agrobacterium tumefaciens vector

Cotyledons from 6-day-old Lotus corniculatus cv. Bokor seedlings, transversally cut into two halves, were capa­ble of regenerating buds without intervening callus formation. The explants were co-cultivated with the Agrobacterium tumefaciens LBA4404/pTOK233 superbinary vector carrying the uidA-intron...

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Bibliographic Details
Main Authors: Nikolić Radomirka, Mitić Nevena, Ninković Slavica, Nešković Mirjana
Format: Article
Language:English
Published: University of Belgrade, University of Novi Sad 2007-01-01
Series:Archives of Biological Sciences
Subjects:
Agrobacterium tumefaciens
genetic transformation
β-glucuronidase
hygromycin B
Lotus corniculatus
Online Access:http://www.doiserbia.nb.rs/img/doi/0354-4664/2007/0354-46640704311N.pdf

Internet

http://www.doiserbia.nb.rs/img/doi/0354-4664/2007/0354-46640704311N.pdf

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