Accuracy of microbial community diversity estimated by closed- and open-reference OTUs
Next-generation sequencing of 16S ribosomal RNA is widely used to survey microbial communities. Sequences are typically assigned to Operational Taxonomic Units (OTUs). Closed- and open-reference OTU assignment matches reads to a reference database at 97% identity (closed), then clusters unmatched re...
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| Format: | Article |
| Language: | English |
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PeerJ Inc.
2017-10-01
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| Series: | PeerJ |
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| Online Access: | https://peerj.com/articles/3889.pdf |