Potassium bisperoxo (1,10-phenanthroline) oxovanadate suppresses proliferation of hippocampal neuronal cell lines by increasing DNA methyltransferases

• Main Authors: Xiao-Li Tian, Shu-Yuan Jiang, Xiao-Lu Zhang, Jie Yang, Jun-He Cui, Xiao-Lei Liu, Ke-Rui Gong, Shao-Chun Yan, Chun-Yang Zhang, Guo Shao
• Format: Article
• Language: English
• Published: Wolters Kluwer Medknow Publications 2019-01-01
• Series: Neural Regeneration Research
• Subjects: nerve regeneration; hippocampal neurons; potassium bisperoxo (1; 10-phenanthroline) oxovanadate; DNA methyltransferase; p21; HT22 cell; cell cycle; immunoblotting; DNA methylation; neural regeneration
• Online Access: http://www.nrronline.org/article.asp?issn=1673-5374;year=2019;volume=14;issue=5;spage=826;epage=833;aulast=Tian

Bisperoxo (1,10-phenanthroline) oxovanadate (BpV) can reportedly block the cell cycle. The present study examined whether BpV alters gene expression by affecting DNA methyltransferases (DNMTs), which would impact the cell cycle. Immortalized mouse hippocampal neuronal precursor cells (HT22) were treated with 0.3 or 3 μM BpV. Proliferation, morphology, and viability of HT22 cells were detected with an IncuCyte real-time video imaging system or inverted microscope and 3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethonyphenol)-2-(4-sulfophenyl)-2H-tetrazolium, respectively. mRNA and protein expression of DNMTs and p21 in HT22 cells was detected by real-time polymerase chain reaction and immunoblotting, respectively. In addition, DNMT activity was measured with an enzyme-linked immunosorbent assay. Effects of BpV on the cell cycle were analyzed using flow cytometry. Results demonstrated that treatment with 0.3 μM BpV did not affect cell proliferation, morphology, or viability; however, treatment with 3 μM BpV decreased cell viability, increased expression of both DNMT3B mRNA and protein, and inhibited the proliferation of HT22 cells; and 3 μM BpV also blocked the cell cycle and increased expression of the regulatory factor p21 by increasing DNMT expression in mouse hippocampal neurons.