Characterization of macrophage infiltration and polarization by double fluorescence immunostaining in mouse colonic mucosa

Summary: We recently characterized the association between DNA damage and immunoresponse in vivo in colonic mucosa of mice infected with a Salmonella Typhimurium strain expressing a genotoxin, known as typhoid toxin. In this protocol, we describe how to assess the extent and features of infiltrating...

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Bibliographic Details
Main Authors: María López Chiloeches, Anna Bergonzini, Teresa Frisan, Océane C.B. Martin
Format: Article
Language:English
Published: Elsevier 2021-12-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721005396