A Fluorescent Probe for the Specific Staining of Cysteine Containing Proteins and Thioredoxin Reductase in SDS-PAGE

A Fluorescent Probe for the Specific Staining of Cysteine Containing Proteins and Thioredoxin Reductase in SDS-PAGE

A naphthalimide-based fluorescent probe, Nap-I, with iodoacetamide as the alkylating group, has been synthesized, and its specific fluorescent staining of proteins containing cysteine (Cys) and selenocysteine (Sec) residues in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) has...

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Bibliographic Details
Main Authors: Yuning Liu, Yanan Yu, Qingshi Meng, Xueting Jia, Jiawei Zhu, Chaohua Tang, Qingyu Zhao, Xiaohui Feng, Junmin Zhang
Format: Article
Language:English
Published: MDPI AG 2021-04-01
Series:Biosensors
Subjects:
fluorescent probe
thiol
thioredoxin reductase
SDS-PAGE
labeling
low pH
Online Access:https://www.mdpi.com/2079-6374/11/5/132

Internet

https://www.mdpi.com/2079-6374/11/5/132

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