Cloning of the Bacillus thuringiensis serovar sotto chitinase (Schi) gene and characterization of its protein

Chitinase plays a positive role in the pathogenicity of Bacillus thuringiensis to insect pests. We used touchdown PCR to clone the chitinase (Schi) gene from Bacillus thuringiensis serovar sotto (Bt sotto) chromosomal DNA. Our DNA sequencing analysis revealed that the Bt sotto Schi gene consists of...

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Bibliographic Details
Main Authors: Wan-Fang Zhong, Ji-Chao Fang, Ping-Zhong Cai, Wen-Zhao Yan, Jie Wu, Hui-Fang Guo
Format: Article
Language:English
Published: Sociedade Brasileira de Genética 2005-12-01
Series:Genetics and Molecular Biology
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500026