CRISPR-Mediated Integration of Large Gene Cassettes Using AAV Donor Vectors
The CRISPR/Cas9 system has recently been shown to facilitate high levels of precise genome editing using adeno-associated viral (AAV) vectors to serve as donor template DNA during homologous recombination (HR). However, the maximum AAV packaging capacity of ∼4.5 kb limits the donor size. Here, we ov...
Main Authors: | , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2017-07-01
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Series: | Cell Reports |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124717308951 |