The pseudogene derived from long non-coding RNA DUXAP10 promotes colorectal cancer cell growth through epigenetically silencing of p21 and PTEN
Abstract Recently, substantial evidence has demonstrated that pseudogene derived lncRNAs are crucial regulators of cancer development and progression. DUXAP10,a pseudogene derived long non-coding RNA(lncRNA), is overexpression in colorectal cancer (CRC), but its expression pattern, biological functi...
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doaj-1373bd91eb3444db874ccc0ed059aa072020-12-08T01:45:58ZengNature Publishing GroupScientific Reports2045-23222017-08-017111110.1038/s41598-017-07954-7The pseudogene derived from long non-coding RNA DUXAP10 promotes colorectal cancer cell growth through epigenetically silencing of p21 and PTENYifan Lian0Yetao Xu1Chuanxing Xiao2Rui Xia3Huangbo Gong4Peng Yang5Tao Chen6Dongdong Wu7Zeling Cai8Jianping Zhang9Keming Wang10Department of Gastroenterology, Zhongshan Hospital affiliated to Xiamen UniversityDepartment of Obstetrics and Gynecology, the First Affiliated Hospital of Nanjing Medical UniversityDepartment of Gastroenterology, Zhongshan Hospital affiliated to Xiamen UniversityDepartment of Laboratory, Nanjing Chest HospitalDepartment of General Surgery, Second Affiliated Hospital, Nanjing Medical UniversityDepartment of General Surgery, Second Affiliated Hospital, Nanjing Medical UniversityDepartment of General Surgery, Second Affiliated Hospital, Nanjing Medical UniversityDepartment of General Surgery, Second Affiliated Hospital, Nanjing Medical UniversityDepartment of General Surgery, Second Affiliated Hospital, Nanjing Medical UniversityDepartment of General Surgery, Second Affiliated Hospital, Nanjing Medical UniversityDepartment of Oncology, Second Affiliated Hospital, Nanjing Medical UniversityAbstract Recently, substantial evidence has demonstrated that pseudogene derived lncRNAs are crucial regulators of cancer development and progression. DUXAP10,a pseudogene derived long non-coding RNA(lncRNA), is overexpression in colorectal cancer (CRC), but its expression pattern, biological function and underlying mechanism in CRC is still undetermined. In this study, we observed that DUXAP10 was up-regulated in CRC tissues which was positively correlated with advanced pathological stages, larger tumor sizes and lymph node metastasis. Additionally, knockdown of DUXAP10 inhibited cell proliferation, induced cell apoptosis and increase the number of G0/G1 cells significantly in the HCT116 and SW480 cell lines. Moreover, DUXAP10 silencing inhibited tumor growth in vivo. Further mechanism study showed that, by binding to histone demethylase lysine-specific demethylase 1 (LSD1), DUXAP10 promote CRC cell growth and reduced cell apoptosis through silencing the expression of p21 and phosphatase and tensin homolog (PTEN) tumor suppressor. Our findings suggested that the pseudogene-derived from lncRNA DUXAP10 promotes the biological progression of CRC and is likely to be a potential therapeutic target for CRC intervention.https://doi.org/10.1038/s41598-017-07954-7 |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yifan Lian Yetao Xu Chuanxing Xiao Rui Xia Huangbo Gong Peng Yang Tao Chen Dongdong Wu Zeling Cai Jianping Zhang Keming Wang |
spellingShingle |
Yifan Lian Yetao Xu Chuanxing Xiao Rui Xia Huangbo Gong Peng Yang Tao Chen Dongdong Wu Zeling Cai Jianping Zhang Keming Wang The pseudogene derived from long non-coding RNA DUXAP10 promotes colorectal cancer cell growth through epigenetically silencing of p21 and PTEN Scientific Reports |
author_facet |
Yifan Lian Yetao Xu Chuanxing Xiao Rui Xia Huangbo Gong Peng Yang Tao Chen Dongdong Wu Zeling Cai Jianping Zhang Keming Wang |
author_sort |
Yifan Lian |
title |
The pseudogene derived from long non-coding RNA DUXAP10 promotes colorectal cancer cell growth through epigenetically silencing of p21 and PTEN |
title_short |
The pseudogene derived from long non-coding RNA DUXAP10 promotes colorectal cancer cell growth through epigenetically silencing of p21 and PTEN |
title_full |
The pseudogene derived from long non-coding RNA DUXAP10 promotes colorectal cancer cell growth through epigenetically silencing of p21 and PTEN |
title_fullStr |
The pseudogene derived from long non-coding RNA DUXAP10 promotes colorectal cancer cell growth through epigenetically silencing of p21 and PTEN |
title_full_unstemmed |
The pseudogene derived from long non-coding RNA DUXAP10 promotes colorectal cancer cell growth through epigenetically silencing of p21 and PTEN |
title_sort |
pseudogene derived from long non-coding rna duxap10 promotes colorectal cancer cell growth through epigenetically silencing of p21 and pten |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2017-08-01 |
description |
Abstract Recently, substantial evidence has demonstrated that pseudogene derived lncRNAs are crucial regulators of cancer development and progression. DUXAP10,a pseudogene derived long non-coding RNA(lncRNA), is overexpression in colorectal cancer (CRC), but its expression pattern, biological function and underlying mechanism in CRC is still undetermined. In this study, we observed that DUXAP10 was up-regulated in CRC tissues which was positively correlated with advanced pathological stages, larger tumor sizes and lymph node metastasis. Additionally, knockdown of DUXAP10 inhibited cell proliferation, induced cell apoptosis and increase the number of G0/G1 cells significantly in the HCT116 and SW480 cell lines. Moreover, DUXAP10 silencing inhibited tumor growth in vivo. Further mechanism study showed that, by binding to histone demethylase lysine-specific demethylase 1 (LSD1), DUXAP10 promote CRC cell growth and reduced cell apoptosis through silencing the expression of p21 and phosphatase and tensin homolog (PTEN) tumor suppressor. Our findings suggested that the pseudogene-derived from lncRNA DUXAP10 promotes the biological progression of CRC and is likely to be a potential therapeutic target for CRC intervention. |
url |
https://doi.org/10.1038/s41598-017-07954-7 |
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