Internal control for real-time polymerase chain reaction based on MS2 bacteriophage for RNA viruses diagnostics

Internal control for real-time polymerase chain reaction based on MS2 bacteriophage for RNA viruses diagnostics

BACKGROUND Real-time reverse transcription polymerase chain reaction (RT-PCR) is routinely used to detect viral infections. In Brazil, it is mandatory the use of nucleic acid tests to detect hepatitis C virus (HCV), hepatitis B virus and human immunodeficiency virus in blood banks because of the im...

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Bibliographic Details
Main Authors: Miriam Ribas Zambenedetti, Daniela Parada Pavoni, Andreia Cristine Dallabona, Alejandro Correa Dominguez, Celina de Oliveira Poersch, Stenio Perdigão Fragoso, Marco Aurélio Krieger
Format: Article
Language:English
Published: Instituto Oswaldo Cruz, Ministério da Saúde
Series:Memórias do Instituto Oswaldo Cruz.
Subjects:
diagnostics
Real-time PCR
internal control
HCV
RNA viruses
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762017000500339&lng=en&tlng=en

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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762017000500339&lng=en&tlng=en

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