Culture of Keratinocytes for Transplantation without the Need of Feeder Layer Cells

Patients with large burn wounds have a limited amount of healthy donor skin. An alternative for the autologous skin graft is transplantation with autologous keratinocytes. Conventionally, the keratinocytes are cultured with mouse feeder layer cells in medium containing fetal calf serum (FCS) to obta...

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Bibliographic Details
Main Authors: Neeltje A. Coolen, Michelle Verkerk, Linda Reijnen, Marcel Vlig, Antoon J. Van Den Bogaerdt, Melanie Breetveld, Susan Gibbs, Esther Middelkoop, Magda M. W. Ulrich
Format: Article
Language:English
Published: SAGE Publishing 2007-07-01
Series:Cell Transplantation
Online Access:https://doi.org/10.3727/000000007783465046