Excision of Nucleopolyhedrovirus Form Transgenic Silkworm Using the CRISPR/Cas9 System

Excision of Nucleopolyhedrovirus Form Transgenic Silkworm Using the CRISPR/Cas9 System

The CRISPR/Cas9-mediated genome engineering has been shown to efficiently suppress infection by disrupting genes of the pathogen. We recently constructed transgenic lines expressing CRISPR/Cas9 and the double sgRNA target Bombyx mori nucleopolyhedrovirus (BmNPV) immediate early-1 (ie-1) gene in the...

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Bibliographic Details
Main Authors: Zhanqi Dong, Feifan Dong, Xinbo Yu, Liang Huang, Yaming Jiang, Zhigang Hu, Peng Chen, Cheng Lu, Minhui Pan
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-02-01
Series:Frontiers in Microbiology
Subjects:
Bombyx mori
BmNPV
CRISPR/Cas9
transgenic
antiviral therapy
Online Access:http://journal.frontiersin.org/article/10.3389/fmicb.2018.00209/full

Internet

http://journal.frontiersin.org/article/10.3389/fmicb.2018.00209/full

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