Water-Soluble Polysaccharides from <i>Ephedra alata</i> Stems: Structural Characterization, Functional Properties, and Antioxidant Activity
In this study, the physicochemical characterization, functional properties, and antioxidant activity of polysaccharides extracted from <i>Ephedra alata</i> (EAP) were investigated. EAP were extracted in water during 3 h with a liquid/solid ratio of 5 in a water bath at 90 °C. The structu...
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doaj-1460798f30324347b3bad14d6e58f7962020-11-25T03:10:03ZengMDPI AGMolecules1420-30492020-05-01252210221010.3390/molecules25092210Water-Soluble Polysaccharides from <i>Ephedra alata</i> Stems: Structural Characterization, Functional Properties, and Antioxidant ActivityLeila Soua0Mohamed Koubaa1Francisco J. Barba2Jawhar Fakhfakh3Hanen Kolsi Ghamgui4Semia Ellouz Chaabouni5Laboratory for the Improvement of Plants and Valorization of Agroresources, National School of Engineering of Sfax (ENIS), University of Sfax, Sfax 3038, TunisiaESCOM, UTC, EA 4297 TIMR, 1 Allée du Réseau Jean-Marie Buckmaster, 60200 Compiègne, FranceNutrition and Food Science Area, Preventive Medicine and Public Health, Food Science, Toxicology and Forensic Medicine Department, Universitat de València, Faculty of Pharmacy, Avda, Vicent Andrés Estellés, s/n, Burjassot, 46100 València, SpainLaboratory of Organic Chemistry, Natural Substances section, Faculty of Sciences of Sfax, PB 802, Sfax 3018, TunisiaLaboratory for the Improvement of Plants and Valorization of Agroresources, National School of Engineering of Sfax (ENIS), University of Sfax, Sfax 3038, TunisiaLaboratory for the Improvement of Plants and Valorization of Agroresources, National School of Engineering of Sfax (ENIS), University of Sfax, Sfax 3038, TunisiaIn this study, the physicochemical characterization, functional properties, and antioxidant activity of polysaccharides extracted from <i>Ephedra alata</i> (EAP) were investigated. EAP were extracted in water during 3 h with a liquid/solid ratio of 5 in a water bath at 90 °C. The structure of the extracted EAP was examined by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and gas chromatography-mass spectrometry (GC-MS). The functional properties and biochemical activities of EAP were determined. The chemical analysis revealed that the contents of carbohydrates, uronic acid, and proteins were 73.24% ± 1.24%, 6.82% ± 0.57%, and 6.56% ± 0.36%, respectively. The results showed that the extracted EAP essentially contain three functional groups: C=O, C-H, and O-H. SEM images showed that EAP present numerous high porosity particles. The monosaccharide composition revealed a polymer composed of glucose (43.1%), galactose (36.4%), mannose (14.9%), arabinose (3.7%), and gluconic acid (1.7%). EAP showed interesting functional properties (solubility, oil holding capacity, foaming and emulsion properties). Finally, the results revealed that EAP displayed excellent antihypertensive and antioxidant activities. Overall, EAP present a promising natural source of food additives, antioxidants, and antihypertensive agents.https://www.mdpi.com/1420-3049/25/9/2210<i>Ephedra alata</i>polysaccharidesphysicochemical characterizationfunctional propertiesantioxidant activities |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Leila Soua Mohamed Koubaa Francisco J. Barba Jawhar Fakhfakh Hanen Kolsi Ghamgui Semia Ellouz Chaabouni |
spellingShingle |
Leila Soua Mohamed Koubaa Francisco J. Barba Jawhar Fakhfakh Hanen Kolsi Ghamgui Semia Ellouz Chaabouni Water-Soluble Polysaccharides from <i>Ephedra alata</i> Stems: Structural Characterization, Functional Properties, and Antioxidant Activity Molecules <i>Ephedra alata</i> polysaccharides physicochemical characterization functional properties antioxidant activities |
author_facet |
Leila Soua Mohamed Koubaa Francisco J. Barba Jawhar Fakhfakh Hanen Kolsi Ghamgui Semia Ellouz Chaabouni |
author_sort |
Leila Soua |
title |
Water-Soluble Polysaccharides from <i>Ephedra alata</i> Stems: Structural Characterization, Functional Properties, and Antioxidant Activity |
title_short |
Water-Soluble Polysaccharides from <i>Ephedra alata</i> Stems: Structural Characterization, Functional Properties, and Antioxidant Activity |
title_full |
Water-Soluble Polysaccharides from <i>Ephedra alata</i> Stems: Structural Characterization, Functional Properties, and Antioxidant Activity |
title_fullStr |
Water-Soluble Polysaccharides from <i>Ephedra alata</i> Stems: Structural Characterization, Functional Properties, and Antioxidant Activity |
title_full_unstemmed |
Water-Soluble Polysaccharides from <i>Ephedra alata</i> Stems: Structural Characterization, Functional Properties, and Antioxidant Activity |
title_sort |
water-soluble polysaccharides from <i>ephedra alata</i> stems: structural characterization, functional properties, and antioxidant activity |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2020-05-01 |
description |
In this study, the physicochemical characterization, functional properties, and antioxidant activity of polysaccharides extracted from <i>Ephedra alata</i> (EAP) were investigated. EAP were extracted in water during 3 h with a liquid/solid ratio of 5 in a water bath at 90 °C. The structure of the extracted EAP was examined by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and gas chromatography-mass spectrometry (GC-MS). The functional properties and biochemical activities of EAP were determined. The chemical analysis revealed that the contents of carbohydrates, uronic acid, and proteins were 73.24% ± 1.24%, 6.82% ± 0.57%, and 6.56% ± 0.36%, respectively. The results showed that the extracted EAP essentially contain three functional groups: C=O, C-H, and O-H. SEM images showed that EAP present numerous high porosity particles. The monosaccharide composition revealed a polymer composed of glucose (43.1%), galactose (36.4%), mannose (14.9%), arabinose (3.7%), and gluconic acid (1.7%). EAP showed interesting functional properties (solubility, oil holding capacity, foaming and emulsion properties). Finally, the results revealed that EAP displayed excellent antihypertensive and antioxidant activities. Overall, EAP present a promising natural source of food additives, antioxidants, and antihypertensive agents. |
topic |
<i>Ephedra alata</i> polysaccharides physicochemical characterization functional properties antioxidant activities |
url |
https://www.mdpi.com/1420-3049/25/9/2210 |
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