Biosynthesis of Random-Homo Block Copolymer Poly[Glycolate-ran-3-Hydroxybutyrate (3HB)]-b-Poly(3HB) Using Sequence-Regulating Chimeric Polyhydroxyalkanoate Synthase in Escherichia coli

Biosynthesis of Random-Homo Block Copolymer Poly[Glycolate-ran-3-Hydroxybutyrate (3HB)]-b-Poly(3HB) Using Sequence-Regulating Chimeric Polyhydroxyalkanoate Synthase in Escherichia coli

Glycolate (GL)-containing polyhydroxyalkanoate (PHA) was synthesized in Escherichia coli expressing the engineered chimeric PHA synthase PhaCAR and coenzyme A transferase. The cells produced poly[GL-co-3-hydroxybutyrate (3HB)] with the supplementation of GL and 3HB, thus demonstrating that PhaCAR is...

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Bibliographic Details
Main Authors: Shuzo Arai, Sayaka Sakakibara, Robin Mareschal, Toshihiko Ooi, Manfred Zinn, Ken’ichiro Matsumoto
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-12-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
sequence regulation
block copolymer
engineered PHA synthase
polyglycolic acid
chimeric enzyme
Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2020.612991/full

Internet

https://www.frontiersin.org/articles/10.3389/fbioe.2020.612991/full

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