Direct infusion-SIM as fast and robust method for absolute protein quantification in complex samples

Direct infusion-SIM as fast and robust method for absolute protein quantification in complex samples

Relative and absolute quantification of proteins in biological and clinical samples are common approaches in proteomics. Until now, targeted protein quantification is mainly performed using a combination of HPLC-based peptide separation and selected reaction monitoring on triple quadrupole mass spec...

Full description

Bibliographic Details
Main Authors: Christina Looße, Sara Galozzi, Linde Debor, Mattijs K. Julsing, Bruno Bühler, Andreas Schmid, Katalin Barkovits, Thorsten Müller, Katrin Marcus
Format: Article
Language:English
Published: Elsevier 2015-06-01
Series:EuPA Open Proteomics
Subjects:
Direct infusion
Quantification
Single ion monitoring (SIM)
Q Exactive
Cytochrome P450 (CYP)
Online Access:http://www.sciencedirect.com/science/article/pii/S2212968515000070

Internet

http://www.sciencedirect.com/science/article/pii/S2212968515000070

Similar Items