Genome editing and transcriptional repression in Pseudomonas putida KT2440 via the type II CRISPR system

Genome editing and transcriptional repression in Pseudomonas putida KT2440 via the type II CRISPR system

Abstract Background The soil bacterium Pseudomonas putida KT2440 is a “generally recognized as safe”-certified strain with robust property and versatile metabolism. Thus, it is an ideal candidate for synthetic biology, biodegradation, and other biotechnology applications. The known genome editing ap...

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Bibliographic Details
Main Authors: Jun Sun, Qingzhuo Wang, Yu Jiang, Zhiqiang Wen, Lirong Yang, Jianping Wu, Sheng Yang
Format: Article
Language:English
Published: BMC 2018-03-01
Series:Microbial Cell Factories
Subjects:
CRISPR–Cas9 system
Pseudomonas putida KT2440
Genome editing
Single nucleotide mutation
Transcriptional engineering
CRISPR–Cpf1 system
Online Access:http://link.springer.com/article/10.1186/s12934-018-0887-x

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http://link.springer.com/article/10.1186/s12934-018-0887-x

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