Re-evaluation of the 3 alpha-hydroxysteroid dehydrogenase assay for total bile acids in bile.
A review of the 3 alpha-hydroxysteroid dehydrogenase method for determining the concentration of total bile acids in bile is described. The optimum conditions for the assay were established with respect to pH, temperature, incubation time, amount of NAD+, and units of enzyme activity required to obt...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1978-09-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520407096 |