Analysis of Mammalian Cell Proliferation and Macromolecule Synthesis Using Deuterated Water and Gas Chromatography-Mass Spectrometry

Analysis of Mammalian Cell Proliferation and Macromolecule Synthesis Using Deuterated Water and Gas Chromatography-Mass Spectrometry

Deuterated water (2H2O), a stable isotopic tracer, provides a convenient and reliable way to label multiple cellular biomass components (macromolecules), thus permitting the calculation of their synthesis rates. Here, we have combined 2H2O labelling, GC-MS analysis and a novel cell fractionation met...

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Bibliographic Details
Main Authors: Victoria C. Foletta, Michelle Palmieri, Joachim Kloehn, Shaun Mason, Stephen F. Previs, Malcolm J. McConville, Oliver M. Sieber, Clinton R. Bruce, Greg M. Kowalski
Format: Article
Language:English
Published: MDPI AG 2016-10-01
Series:Metabolites
Subjects:
deuterated water
biomass
GC-MS
stable isotopes
C2C12
colon cancer
protein synthesis
lipogenesis
DNA synthesis
Online Access:http://www.mdpi.com/2218-1989/6/4/34

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http://www.mdpi.com/2218-1989/6/4/34

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