Protective Effect of SIRT1 Activator on the Knee With Osteoarthritis
Osteoarthritis (OA), one of the most common chronic musculoskeletal disorders, is deemed to be correlated with aging. The SIRT1 activator, resveratrol, acts as a crucial regulator of aging and may have a potential therapeutic effect on OA. Rabbit OA models were established through destabilized media...
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Frontiers Media S.A.
2021-04-01
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Series: | Frontiers in Physiology |
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Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2021.661852/full |
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doaj-15995444346d4264a14ae93d81247046 |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhenquan Zhou Zhenquan Zhou Zhenquan Zhou Zhenhan Deng Zhenhan Deng Zhenhan Deng Zhenhan Deng Zhenhan Deng Yuwei Liu Yizi Zheng Shiwei Yang Shiwei Yang Wei Lu Deming Xiao Weimin Zhu Weimin Zhu Weimin Zhu Weimin Zhu Weimin Zhu |
spellingShingle |
Zhenquan Zhou Zhenquan Zhou Zhenquan Zhou Zhenhan Deng Zhenhan Deng Zhenhan Deng Zhenhan Deng Zhenhan Deng Yuwei Liu Yizi Zheng Shiwei Yang Shiwei Yang Wei Lu Deming Xiao Weimin Zhu Weimin Zhu Weimin Zhu Weimin Zhu Weimin Zhu Protective Effect of SIRT1 Activator on the Knee With Osteoarthritis Frontiers in Physiology osteoarthritis resveratrol SIRT1 p53 cartilage Micro-CT |
author_facet |
Zhenquan Zhou Zhenquan Zhou Zhenquan Zhou Zhenhan Deng Zhenhan Deng Zhenhan Deng Zhenhan Deng Zhenhan Deng Yuwei Liu Yizi Zheng Shiwei Yang Shiwei Yang Wei Lu Deming Xiao Weimin Zhu Weimin Zhu Weimin Zhu Weimin Zhu Weimin Zhu |
author_sort |
Zhenquan Zhou |
title |
Protective Effect of SIRT1 Activator on the Knee With Osteoarthritis |
title_short |
Protective Effect of SIRT1 Activator on the Knee With Osteoarthritis |
title_full |
Protective Effect of SIRT1 Activator on the Knee With Osteoarthritis |
title_fullStr |
Protective Effect of SIRT1 Activator on the Knee With Osteoarthritis |
title_full_unstemmed |
Protective Effect of SIRT1 Activator on the Knee With Osteoarthritis |
title_sort |
protective effect of sirt1 activator on the knee with osteoarthritis |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Physiology |
issn |
1664-042X |
publishDate |
2021-04-01 |
description |
Osteoarthritis (OA), one of the most common chronic musculoskeletal disorders, is deemed to be correlated with aging. The SIRT1 activator, resveratrol, acts as a crucial regulator of aging and may have a potential therapeutic effect on OA. Rabbit OA models were established through destabilized medial meniscus surgery. A total of 40 healthy male New Zealand rabbits were divided into five groups: control group (sham operation), OA group, as well as low dose (LD), middle dose (MD), and high dose (HD) resveratrol-treated OA groups. 6 weeks after operation, 0.8 ml of normal saline was injected into the knee joints every other day in the control and OA groups, and 0.8 ml of 5, 10, and 15 μmol/L resveratrol was injected into the knee joints every other day in the LD, MD, and HD group, respectively. The rabbits were sacrificed 2 weeks after medication, and the articular cartilage of the knee joint was collected for Micro-CT, histology and Western blot analysis. Obvious articular cartilage lesion and joint space narrowing were detected in the OA group. Compared with the OA group, less osteoarthritic changes were observed in the MD and HD groups. The MD and HD groups had significantly lower bone volume fraction, trabecular number and Mankin scores than the LD and OA groups (p < 0.05). No significant difference was found between the OA and LD groups (p > 0.05). The expressions of SIRT1 and p53 detected by western blot were consistent with the aforementioned findings. Therefore, resveratrol can activate the SIRT1 gene to play a protective role in the OA process by inhibiting chondrocyte apoptosis, trabecular bone number increasing of the subchondral bone, as well as elevation of bone density. It demonstrated the importance of SIRT1 in maintaining articular cartilage health and provided a promising therapeutic intervention in the treatment of OA. |
topic |
osteoarthritis resveratrol SIRT1 p53 cartilage Micro-CT |
url |
https://www.frontiersin.org/articles/10.3389/fphys.2021.661852/full |
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doaj-15995444346d4264a14ae93d812470462021-04-13T06:21:44ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2021-04-011210.3389/fphys.2021.661852661852Protective Effect of SIRT1 Activator on the Knee With OsteoarthritisZhenquan Zhou0Zhenquan Zhou1Zhenquan Zhou2Zhenhan Deng3Zhenhan Deng4Zhenhan Deng5Zhenhan Deng6Zhenhan Deng7Yuwei Liu8Yizi Zheng9Shiwei Yang10Shiwei Yang11Wei Lu12Deming Xiao13Weimin Zhu14Weimin Zhu15Weimin Zhu16Weimin Zhu17Weimin Zhu18Department of Sports Medicine, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital, Shenzhen, ChinaDepartment of Orthopaedics, Shenzhen Hospital of Southern Medical University, Shenzhen, ChinaClinical Medical College, Guangzhou Medical University, Guangzhou, ChinaDepartment of Sports Medicine, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital, Shenzhen, ChinaClinical Medical College, Guangzhou Medical University, Guangzhou, ChinaClinical Medical College, Shenzhen University, Shenzhen, ChinaClinical Medical College, Guangxi University of Chinese Medicine, Nanning, ChinaClinical Medical College, Anhui Medical University, Hefei, ChinaDepartment of Sports Medicine, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital, Shenzhen, ChinaDepartment of Thyroid and Breast Surgery, Shenzhen Breast Tumor Research Center for Diagnosis and Treatment, National Standardization Center for Breast Cancer Diagnosis and Treatment, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, ChinaClinical Medical College, Anhui Medical University, Hefei, ChinaTeaching Office, Shenzhen Second People’s Hospital, Shenzhen, ChinaDepartment of Sports Medicine, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital, Shenzhen, ChinaClinical Medical College, Guangzhou Medical University, Guangzhou, ChinaDepartment of Sports Medicine, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital, Shenzhen, ChinaClinical Medical College, Guangzhou Medical University, Guangzhou, ChinaClinical Medical College, Shenzhen University, Shenzhen, ChinaClinical Medical College, Guangxi University of Chinese Medicine, Nanning, ChinaClinical Medical College, Anhui Medical University, Hefei, ChinaOsteoarthritis (OA), one of the most common chronic musculoskeletal disorders, is deemed to be correlated with aging. The SIRT1 activator, resveratrol, acts as a crucial regulator of aging and may have a potential therapeutic effect on OA. Rabbit OA models were established through destabilized medial meniscus surgery. A total of 40 healthy male New Zealand rabbits were divided into five groups: control group (sham operation), OA group, as well as low dose (LD), middle dose (MD), and high dose (HD) resveratrol-treated OA groups. 6 weeks after operation, 0.8 ml of normal saline was injected into the knee joints every other day in the control and OA groups, and 0.8 ml of 5, 10, and 15 μmol/L resveratrol was injected into the knee joints every other day in the LD, MD, and HD group, respectively. The rabbits were sacrificed 2 weeks after medication, and the articular cartilage of the knee joint was collected for Micro-CT, histology and Western blot analysis. Obvious articular cartilage lesion and joint space narrowing were detected in the OA group. Compared with the OA group, less osteoarthritic changes were observed in the MD and HD groups. The MD and HD groups had significantly lower bone volume fraction, trabecular number and Mankin scores than the LD and OA groups (p < 0.05). No significant difference was found between the OA and LD groups (p > 0.05). The expressions of SIRT1 and p53 detected by western blot were consistent with the aforementioned findings. Therefore, resveratrol can activate the SIRT1 gene to play a protective role in the OA process by inhibiting chondrocyte apoptosis, trabecular bone number increasing of the subchondral bone, as well as elevation of bone density. It demonstrated the importance of SIRT1 in maintaining articular cartilage health and provided a promising therapeutic intervention in the treatment of OA.https://www.frontiersin.org/articles/10.3389/fphys.2021.661852/fullosteoarthritisresveratrolSIRT1p53cartilageMicro-CT |