Induction of Plasmodium falciparum-specific CD4+ T cells and memory B cells in Gabonese children vaccinated with RTS,S/AS01(E) and RTS,S/AS02(D).
The recombinant circumsporozoite protein (CS) based vaccine, RTS,S, confers protection against Plasmodium falciparum infection in controlled challenge trials and in field studies. The RTS,S recombinant antigen has been formulated with two adjuvant systems, AS01 and AS02, which have both been shown t...
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doaj-15c7ade9e728478f8dac448f92278a4d2020-11-25T00:07:59ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-04-0164e1855910.1371/journal.pone.0018559Induction of Plasmodium falciparum-specific CD4+ T cells and memory B cells in Gabonese children vaccinated with RTS,S/AS01(E) and RTS,S/AS02(D).Selidji T AgnandjiRolf FendelMichaël MestréMichel JanssensJohan VekemansJana HeldFerdinand GnansounouSonja HaertleIsabel von GlasenappSunny OyakhiromeLudovic MewonoPhilippe MorisMarc LievensMarie-Ange DemoitiePatrice M DuboisTonya VillafanaErik JongertAurelie OlivierJoe CohenMeral EsenPeter G KremsnerBertrand LellBenjamin MordmüllerThe recombinant circumsporozoite protein (CS) based vaccine, RTS,S, confers protection against Plasmodium falciparum infection in controlled challenge trials and in field studies. The RTS,S recombinant antigen has been formulated with two adjuvant systems, AS01 and AS02, which have both been shown to induce strong specific antibody responses and CD4 T cell responses in adults. As infants and young children are particularly susceptible to malaria infection and constitute the main target population for a malaria vaccine, we have evaluated the induction of adaptive immune responses in young children living in malaria endemic regions following vaccination with RTS,S/AS01(E) and RTS,S/AS02(D). Our data show that a CS-specific memory B cell response is induced one month after the second and third vaccine dose and that CS-specific antibodies and memory B cells persist up to 12 months after the last vaccine injection. Both formulations also induced low but significant amounts of CS-specific IL-2(+) CD4(+) T cells one month after the second and third vaccine dose, upon short-term in vitro stimulation of whole blood cells with peptides covering the entire CS derived sequence in RTS,S. These results provide evidence that both RTS,S/AS01(E) and RTS,S/AS02(D) induced adaptive immune responses including antibodies, circulating memory B cells and CD4(+) T cells directed against P. falciparum CS protein.ClinicalTrials.gov NCT00307021.http://europepmc.org/articles/PMC3073948?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Selidji T Agnandji Rolf Fendel Michaël Mestré Michel Janssens Johan Vekemans Jana Held Ferdinand Gnansounou Sonja Haertle Isabel von Glasenapp Sunny Oyakhirome Ludovic Mewono Philippe Moris Marc Lievens Marie-Ange Demoitie Patrice M Dubois Tonya Villafana Erik Jongert Aurelie Olivier Joe Cohen Meral Esen Peter G Kremsner Bertrand Lell Benjamin Mordmüller |
spellingShingle |
Selidji T Agnandji Rolf Fendel Michaël Mestré Michel Janssens Johan Vekemans Jana Held Ferdinand Gnansounou Sonja Haertle Isabel von Glasenapp Sunny Oyakhirome Ludovic Mewono Philippe Moris Marc Lievens Marie-Ange Demoitie Patrice M Dubois Tonya Villafana Erik Jongert Aurelie Olivier Joe Cohen Meral Esen Peter G Kremsner Bertrand Lell Benjamin Mordmüller Induction of Plasmodium falciparum-specific CD4+ T cells and memory B cells in Gabonese children vaccinated with RTS,S/AS01(E) and RTS,S/AS02(D). PLoS ONE |
author_facet |
Selidji T Agnandji Rolf Fendel Michaël Mestré Michel Janssens Johan Vekemans Jana Held Ferdinand Gnansounou Sonja Haertle Isabel von Glasenapp Sunny Oyakhirome Ludovic Mewono Philippe Moris Marc Lievens Marie-Ange Demoitie Patrice M Dubois Tonya Villafana Erik Jongert Aurelie Olivier Joe Cohen Meral Esen Peter G Kremsner Bertrand Lell Benjamin Mordmüller |
author_sort |
Selidji T Agnandji |
title |
Induction of Plasmodium falciparum-specific CD4+ T cells and memory B cells in Gabonese children vaccinated with RTS,S/AS01(E) and RTS,S/AS02(D). |
title_short |
Induction of Plasmodium falciparum-specific CD4+ T cells and memory B cells in Gabonese children vaccinated with RTS,S/AS01(E) and RTS,S/AS02(D). |
title_full |
Induction of Plasmodium falciparum-specific CD4+ T cells and memory B cells in Gabonese children vaccinated with RTS,S/AS01(E) and RTS,S/AS02(D). |
title_fullStr |
Induction of Plasmodium falciparum-specific CD4+ T cells and memory B cells in Gabonese children vaccinated with RTS,S/AS01(E) and RTS,S/AS02(D). |
title_full_unstemmed |
Induction of Plasmodium falciparum-specific CD4+ T cells and memory B cells in Gabonese children vaccinated with RTS,S/AS01(E) and RTS,S/AS02(D). |
title_sort |
induction of plasmodium falciparum-specific cd4+ t cells and memory b cells in gabonese children vaccinated with rts,s/as01(e) and rts,s/as02(d). |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2011-04-01 |
description |
The recombinant circumsporozoite protein (CS) based vaccine, RTS,S, confers protection against Plasmodium falciparum infection in controlled challenge trials and in field studies. The RTS,S recombinant antigen has been formulated with two adjuvant systems, AS01 and AS02, which have both been shown to induce strong specific antibody responses and CD4 T cell responses in adults. As infants and young children are particularly susceptible to malaria infection and constitute the main target population for a malaria vaccine, we have evaluated the induction of adaptive immune responses in young children living in malaria endemic regions following vaccination with RTS,S/AS01(E) and RTS,S/AS02(D). Our data show that a CS-specific memory B cell response is induced one month after the second and third vaccine dose and that CS-specific antibodies and memory B cells persist up to 12 months after the last vaccine injection. Both formulations also induced low but significant amounts of CS-specific IL-2(+) CD4(+) T cells one month after the second and third vaccine dose, upon short-term in vitro stimulation of whole blood cells with peptides covering the entire CS derived sequence in RTS,S. These results provide evidence that both RTS,S/AS01(E) and RTS,S/AS02(D) induced adaptive immune responses including antibodies, circulating memory B cells and CD4(+) T cells directed against P. falciparum CS protein.ClinicalTrials.gov NCT00307021. |
url |
http://europepmc.org/articles/PMC3073948?pdf=render |
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