Molecular Characterization of TP53 Gene in Human Populations Exposed to Low-Dose Ionizing Radiation
Ionizing radiation, such as that emitted by uranium, may cause mutations and consequently lead to neoplasia in human cells. The TP53 gene acts to maintain genomic integrity and constitutes an important biomarker of susceptibility. The present study investigated the main alterations observed in exons...
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doaj-15d61f5d1cdd4c81a99016e39339eab22020-11-24T22:48:55ZengHindawi LimitedBioMed Research International2314-61332314-61412013-01-01201310.1155/2013/303486303486Molecular Characterization of TP53 Gene in Human Populations Exposed to Low-Dose Ionizing RadiationIgor Brasil-Costa0Dayse O. Alencar1Milene Raiol-Moraes2Igor A. Pessoa3Alexandre W. M. Brito4Schneyder R. Jati5Sidney E. B. Santos6Rommel M. R. Burbano7Ândrea K. C. Ribeiro-dos-Santos8Laboratory of Human and Medical Genetics, Biological Sciences Institute, Federal University of Pará (UFPA), Augusto Correa Street, Number 01, CEP 66075-110 Belém, PA, BrazilLaboratory of Human and Medical Genetics, Biological Sciences Institute, Federal University of Pará (UFPA), Augusto Correa Street, Number 01, CEP 66075-110 Belém, PA, BrazilLaboratory of Human and Medical Genetics, Biological Sciences Institute, Federal University of Pará (UFPA), Augusto Correa Street, Number 01, CEP 66075-110 Belém, PA, BrazilLaboratory of Human and Medical Genetics, Biological Sciences Institute, Federal University of Pará (UFPA), Augusto Correa Street, Number 01, CEP 66075-110 Belém, PA, BrazilHuman Cytogenetics Laboratory, Biological Sciences Institute, Federal University of Pará (UFPA), Augusto Correa Street, Number 01, CEP 66075-110 Belém, PA, BrazilHuman Cytogenetics Laboratory, Biological Sciences Institute, Federal University of Pará (UFPA), Augusto Correa Street, Number 01, CEP 66075-110 Belém, PA, BrazilLaboratory of Human and Medical Genetics, Biological Sciences Institute, Federal University of Pará (UFPA), Augusto Correa Street, Number 01, CEP 66075-110 Belém, PA, BrazilHuman Cytogenetics Laboratory, Biological Sciences Institute, Federal University of Pará (UFPA), Augusto Correa Street, Number 01, CEP 66075-110 Belém, PA, BrazilLaboratory of Human and Medical Genetics, Biological Sciences Institute, Federal University of Pará (UFPA), Augusto Correa Street, Number 01, CEP 66075-110 Belém, PA, BrazilIonizing radiation, such as that emitted by uranium, may cause mutations and consequently lead to neoplasia in human cells. The TP53 gene acts to maintain genomic integrity and constitutes an important biomarker of susceptibility. The present study investigated the main alterations observed in exons 4, 5, 6, 7, and 8 of the TP53 gene and adjacent introns in Amazonian populations exposed to radioactivity. Samples were collected from 163 individuals. Occurrence of the following alterations was observed: (i) a missense exchange in exon 4 (Arg72Pro); (ii) 2 synonymous exchanges, 1 in exon 5 (His179His), and another in exon 6 (Arg213Arg); (iii) 4 intronic exchanges, 3 in intron 7 (C → T at position 13.436; C → T at position 13.491; T → G at position 13.511) and 1 in intron 8 (T → G at position 13.958). Alteration of codon 72 was found to be an important risk factor for cancer development (P=0.024; OR=6.48; CI: 1.29–32.64) when adjusted for age and smoking. Thus, TP53 gene may be an important biomarker for carcinogenesis susceptibility in human populations exposed to ionizing radiation.http://dx.doi.org/10.1155/2013/303486 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Igor Brasil-Costa Dayse O. Alencar Milene Raiol-Moraes Igor A. Pessoa Alexandre W. M. Brito Schneyder R. Jati Sidney E. B. Santos Rommel M. R. Burbano Ândrea K. C. Ribeiro-dos-Santos |
spellingShingle |
Igor Brasil-Costa Dayse O. Alencar Milene Raiol-Moraes Igor A. Pessoa Alexandre W. M. Brito Schneyder R. Jati Sidney E. B. Santos Rommel M. R. Burbano Ândrea K. C. Ribeiro-dos-Santos Molecular Characterization of TP53 Gene in Human Populations Exposed to Low-Dose Ionizing Radiation BioMed Research International |
author_facet |
Igor Brasil-Costa Dayse O. Alencar Milene Raiol-Moraes Igor A. Pessoa Alexandre W. M. Brito Schneyder R. Jati Sidney E. B. Santos Rommel M. R. Burbano Ândrea K. C. Ribeiro-dos-Santos |
author_sort |
Igor Brasil-Costa |
title |
Molecular Characterization of TP53 Gene in Human Populations Exposed to Low-Dose Ionizing Radiation |
title_short |
Molecular Characterization of TP53 Gene in Human Populations Exposed to Low-Dose Ionizing Radiation |
title_full |
Molecular Characterization of TP53 Gene in Human Populations Exposed to Low-Dose Ionizing Radiation |
title_fullStr |
Molecular Characterization of TP53 Gene in Human Populations Exposed to Low-Dose Ionizing Radiation |
title_full_unstemmed |
Molecular Characterization of TP53 Gene in Human Populations Exposed to Low-Dose Ionizing Radiation |
title_sort |
molecular characterization of tp53 gene in human populations exposed to low-dose ionizing radiation |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2013-01-01 |
description |
Ionizing radiation, such as that emitted by uranium, may cause mutations and consequently lead to neoplasia in human cells. The TP53 gene acts to maintain genomic integrity and constitutes an important biomarker of susceptibility. The present study investigated the main alterations observed in exons 4, 5, 6, 7, and 8 of the TP53 gene and adjacent introns in Amazonian populations exposed to radioactivity. Samples were collected from 163 individuals. Occurrence of the following alterations was observed: (i) a missense exchange in exon 4 (Arg72Pro); (ii) 2 synonymous exchanges, 1 in exon 5 (His179His), and another in exon 6 (Arg213Arg); (iii) 4 intronic exchanges, 3 in intron 7 (C → T at position 13.436; C → T at position 13.491; T → G at position 13.511) and 1 in intron 8 (T → G at position 13.958). Alteration of codon 72 was found to be an important risk factor for cancer development (P=0.024; OR=6.48; CI: 1.29–32.64) when adjusted for age and smoking. Thus, TP53 gene may be an important biomarker for carcinogenesis susceptibility in human populations exposed to ionizing radiation. |
url |
http://dx.doi.org/10.1155/2013/303486 |
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