Combining different mRNA capture methods to analyze the transcriptome: analysis of the Xenopus laevis transcriptome.

mRNA sequencing (mRNA-seq) is a commonly used technique to survey gene expression from organisms with fully sequenced genomes. Successful mRNA-seq requires purification of mRNA away from the much more abundant ribosomal RNA, which is typically accomplished by oligo-dT selection. However, mRNAs with...

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Bibliographic Details
Main Authors: Michael D Blower, Ashwini Jambhekar, Dianne S Schwarz, James A Toombs
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3797054?pdf=render