PTO-QuickStep: A Fast and Efficient Method for Cloning Random Mutagenesis Libraries

PTO-QuickStep: A Fast and Efficient Method for Cloning Random Mutagenesis Libraries

QuickStep is a cloning method that allows seamless point integration of a DNA sequence at any position within a target plasmid using only Q5 High-Fidelity DNA Polymerase and DpnI endonuclease. This efficient and cost-effective method consists of two steps: two parallel asymmetric PCRs, followed by a...

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Bibliographic Details
Main Authors: Pawel Jajesniak, Kang Lan Tee, Tuck Seng Wong
Format: Article
Language:English
Published: MDPI AG 2019-08-01
Series:International Journal of Molecular Sciences
Subjects:
cloning
QuickStep
PTO-QuickStep
random mutagenesis
directed evolution
protein engineering
synthetic biology
Online Access:https://www.mdpi.com/1422-0067/20/16/3908

Internet

https://www.mdpi.com/1422-0067/20/16/3908

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