Detection of SARS-CoV-2 RNA by a Multiplex Reverse-Transcription Loop-Mediated Isothermal Amplification Coupled with Melting Curves Analysis

Detection of SARS-CoV-2 RNA by a Multiplex Reverse-Transcription Loop-Mediated Isothermal Amplification Coupled with Melting Curves Analysis

Loop-mediated isothermal amplification (LAMP) is a method of nucleic acid amplification that is more stable and resistant to DNA amplification inhibitors than conventional PCR. LAMP multiplexing with reverse transcription allows for the single-tube amplification of several RNA fragments, including a...

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Bibliographic Details
Main Authors: Igor P. Oscorbin, Georgiy Yu. Shevelev, Ksenia A. Pronyaeva, Andrey A. Stepanov, Darya V. Shamovskaya, Olga V. Mishukova, Dmitrii V. Pyshnyi, Maksim L. Filipenko
Format: Article
Language:English
Published: MDPI AG 2021-05-01
Series:International Journal of Molecular Sciences
Subjects:
SARS-CoV-2
coronavirus
loop-mediated isothermal amplification
LAMP
multiplex amplification
melting curve analysis
Online Access:https://www.mdpi.com/1422-0067/22/11/5743

Internet

https://www.mdpi.com/1422-0067/22/11/5743

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