Parameter-free binarization and skeletonization of fiber networks from confocal image stacks.

We present a method to reconstruct a disordered network of thin biopolymers, such as collagen gels, from three-dimensional (3D) image stacks recorded with a confocal microscope. The method is based on a template matching algorithm that simultaneously performs a binarization and skeletonization of th...

Full description

Bibliographic Details
Main Authors: Patrick Krauss, Claus Metzner, Janina Lange, Nadine Lang, Ben Fabry
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3351466?pdf=render