Fact versus artifact: Avoiding erroneous estimates of sulfated glycosaminoglycan content using the dimethylmethylene blue colorimetric assay for tissue-engineered constructs
The 1,9-dimethylmethylene blue (DMMB) assay is widely used to quantify sulfated glycosaminoglycan (sGAG) contents of engineered tissues, culture media, tissue samples and bodily fluids, but the assay is subject to interference from polyanions such as hyaluronic acid (HA), DNA and RNA. We examined wh...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
AO Research Institute Davos
2015-04-01
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| Series: | European Cells & Materials |
| Subjects: | |
| Online Access: | http://www.ecmjournal.org/journal/papers/vol029/pdf/v029a17.pdf |