Characterisation of Structural Proteins from Chronic Bee Paralysis Virus (CBPV) Using Mass Spectrometry

• Main Authors: Aurore Chevin, Bruno Coutard, Philippe Blanchard, Anne-Sophie Dabert-Gay, Magali Ribière-Chabert, Richard Thiéry
• Format: Article
• Language: English
• Published: MDPI AG 2015-06-01
• Series: Viruses
• Subjects: chronic bee paralysis virus (CBPV); nano-HPLC; MALDI-TOF/TOF; protein identification; hypothetical structural protein (hSP); predicted structural protein (pSP); anti-pSP antibodies
• Online Access: http://www.mdpi.com/1999-4915/7/6/2774

Chronic bee paralysis virus (CBPV) is the etiological agent of chronic paralysis, an infectious and contagious disease in adult honeybees. CBPV is a positive single-stranded RNA virus which contains two major viral RNA fragments. RNA 1 (3674 nt) and RNA 2 (2305 nt) encode three and four putative open reading frames (ORFs), respectively. RNA 1 is thought to encode the viral RNA-dependent RNA polymerase (RdRp) since the amino acid sequence derived from ORF 3 shares similarities with the RdRP of families Nodaviridae and Tombusviridae. The genomic organization of CBPV and in silico analyses have suggested that RNA 1 encodes non-structural proteins, while RNA 2 encodes structural proteins, which are probably encoded by ORFs 2 and 3. In this study, purified CBPV particles were used to characterize virion proteins by mass spectrometry. Several polypeptides corresponding to proteins encoded by ORF 2 and 3 on RNA 2 were detected. Their role in the formation of the viral capsid is discussed.