Workflow for large-scale analysis of melanoma tissue samples

The aim of the present study was to create an optimal workflow for analysing a large cohort of malignant melanoma tissue samples. Samples were lysed with urea and enzymatically digested with trypsin or trypsin/Lys C. Buffer exchange or dilution was used to reduce urea concentration prior to digestio...

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Bibliographic Details
Main Authors: Maria E. Yakovleva, Charlotte Welinder, Yutaka Sugihara, Krzysztof Pawłowski, Melinda Rezeli, Elisabet Wieslander, Johan Malm, György Marko-Varga
Format: Article
Language:English
Published: Elsevier 2015-09-01
Series:EuPA Open Proteomics
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S221296851530012X