Challenging the proposed causes of the PCR plateau phase

Despite the wide-spread use of the polymerase chain reaction (PCR) in various life-science applications, the causes of arrested amplicon generation in late cycles have not been confidently identified. This so-called plateau phase has been attributed to depletion or thermal break-down of primers or n...

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Bibliographic Details
Main Authors: Linda Jansson, Johannes Hedman
Format: Article
Language:English
Published: Elsevier 2019-03-01
Series:Biomolecular Detection and Quantification
Online Access:http://www.sciencedirect.com/science/article/pii/S2214753518300196