Challenging the proposed causes of the PCR plateau phase
Despite the wide-spread use of the polymerase chain reaction (PCR) in various life-science applications, the causes of arrested amplicon generation in late cycles have not been confidently identified. This so-called plateau phase has been attributed to depletion or thermal break-down of primers or n...
Main Authors: | , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2019-03-01
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Series: | Biomolecular Detection and Quantification |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2214753518300196 |