Knocking out Analysis of the CpxP gene using Crispr/Cas9 in Escherichia coli MG1655
Abstract Based on the analysis of cpxP genes among Escherichia coli strains, cpxP gene-targeting short guide RNA (sgRNA) was designed and inserted into the pGL3-MGP-RNA. The donor sequences (MG-HR) for homologous repair were designed and cloned by PCR. MG-HR and pGL3-MGP-RNA were transformed into E....
Main Authors: | , , , , |
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Format: | Article |
Language: | English |
Published: |
SpringerOpen
2020-09-01
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Series: | AMB Express |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s13568-020-01099-z |