Efficient genome editing using CRISPR/Cas9 ribonucleoprotein approach in cultured Medaka fish cells

Efficient genome editing using CRISPR/Cas9 ribonucleoprotein approach in cultured Medaka fish cells

Gene editing with CRISPR/Cas9 is a powerful tool to study the function of target genes. Although this technology has demonstrated wide efficiency in many species, including fertilized zebrafish and medaka fish embryos when microinjected, its application to achieve efficient gene editing in cultured...

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Bibliographic Details
Main Authors: Qizhi Liu, Yongming Yuan, Feng Zhu, Yunhan Hong, Ruowen Ge
Format: Article
Language:English
Published: The Company of Biologists 2018-08-01
Series:Biology Open
Subjects:
Genome editing
CRISPR/Cas9
Medaka
RNP
Electroporation
Online Access:http://bio.biologists.org/content/7/8/bio035170

Internet

http://bio.biologists.org/content/7/8/bio035170

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