Targeted Inhibition of the NUP98-NSD1 Fusion Oncogene in Acute Myeloid Leukemia
NUP98-NSD1-positive acute myeloid leukemia (AML) is a poor prognostic subgroup that is frequently diagnosed in pediatric cytogenetically normal AML. NUP98-NSD1-positive AML often carries additional mutations in genes including <i>FLT3, NRAS, WT1,</i> and <i>MYC</i>. The purpo...
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2020-09-01
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doaj-184604dbfb1d4e118fbac68f2c68efe52020-11-25T03:19:02ZengMDPI AGCancers2072-66942020-09-01122766276610.3390/cancers12102766Targeted Inhibition of the NUP98-NSD1 Fusion Oncogene in Acute Myeloid LeukemiaSagarajit Mohanty0Nidhi Jyotsana1Amit Sharma2Arnold Kloos3Razif Gabdoulline4Basem Othman5Courteney K. Lai6Renate Schottmann7Madhvi Mandhania8Johannes Schmoellerl9Florian Grebien10Euan Ramsay11Anitha Thomas12Hans-Peter Vornlocher13Arnold Ganser14Felicitas Thol15Michael Heuser16Department of Hematology, Hemostasis, Oncology and Stem Cell Transplantation, Hannover Medical School, 30625 Hannover, GermanyDepartment of Biomedical Engineering, Vanderbilt University, Nashville, TN 37235, USADepartment of Hematology, Hemostasis, Oncology and Stem Cell Transplantation, Hannover Medical School, 30625 Hannover, GermanyDepartment of Hematology, Hemostasis, Oncology and Stem Cell Transplantation, Hannover Medical School, 30625 Hannover, GermanyDepartment of Hematology, Hemostasis, Oncology and Stem Cell Transplantation, Hannover Medical School, 30625 Hannover, GermanyDepartment of Hematology, Hemostasis, Oncology and Stem Cell Transplantation, Hannover Medical School, 30625 Hannover, GermanyDepartment of Hematology, Hemostasis, Oncology and Stem Cell Transplantation, Hannover Medical School, 30625 Hannover, GermanyDepartment of Hematology, Hemostasis, Oncology and Stem Cell Transplantation, Hannover Medical School, 30625 Hannover, GermanyNational Centre for Cell Science, Pune 411007, IndiaInstitute for Medical Biochemistry, University of Veterinary Medicine Vienna, 1210 Vienna, AustriaInstitute for Medical Biochemistry, University of Veterinary Medicine Vienna, 1210 Vienna, AustriaPrecision NanoSystems Inc., Vancouver, BC V6P 6T7, CanadaPrecision NanoSystems Inc., Vancouver, BC V6P 6T7, CanadaAxolabsGmbH, 95326 Kulmbach, GermanyDepartment of Hematology, Hemostasis, Oncology and Stem Cell Transplantation, Hannover Medical School, 30625 Hannover, GermanyDepartment of Hematology, Hemostasis, Oncology and Stem Cell Transplantation, Hannover Medical School, 30625 Hannover, GermanyDepartment of Hematology, Hemostasis, Oncology and Stem Cell Transplantation, Hannover Medical School, 30625 Hannover, GermanyNUP98-NSD1-positive acute myeloid leukemia (AML) is a poor prognostic subgroup that is frequently diagnosed in pediatric cytogenetically normal AML. NUP98-NSD1-positive AML often carries additional mutations in genes including <i>FLT3, NRAS, WT1,</i> and <i>MYC</i>. The purpose of our study was to characterize the cooperative potential of the fusion and its associated Neuroblastoma rat sarcoma (NRAS) mutation. By constitutively expressing NUP98-NSD1 and NRASG12D in a syngeneic mouse model and using a patient-derived xenograft (PDX) model from a NUP98-NSD1-positive AML patient, we evaluated the functional role of these genes and tested a novel siRNA formulation that inhibits the oncogenic driver NUP98-NSD1. NUP98-NSD1 transformed murine bone marrow (BM) cells in vitro and induced AML in vivo. While NRASG12D expression was insufficient to transform cells alone, co-expression of NUP98-NSD1 and NRASG12D enhanced the leukemogenicity of NUP98-NSD1. We developed a NUP98-NSD1-targeting siRNA/lipid nanoparticle formulation that significantly prolonged the survival of the PDX mice. Our study demonstrates that mutated NRAS cooperates with NUP98-NSD1 and shows that direct targeting of the fusion can be exploited as a novel treatment strategy in NUP98-NSD1-positive AML patients.https://www.mdpi.com/2072-6694/12/10/2766AMLNUP98-NSD1NRASG12DsiRNAliposome |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sagarajit Mohanty Nidhi Jyotsana Amit Sharma Arnold Kloos Razif Gabdoulline Basem Othman Courteney K. Lai Renate Schottmann Madhvi Mandhania Johannes Schmoellerl Florian Grebien Euan Ramsay Anitha Thomas Hans-Peter Vornlocher Arnold Ganser Felicitas Thol Michael Heuser |
spellingShingle |
Sagarajit Mohanty Nidhi Jyotsana Amit Sharma Arnold Kloos Razif Gabdoulline Basem Othman Courteney K. Lai Renate Schottmann Madhvi Mandhania Johannes Schmoellerl Florian Grebien Euan Ramsay Anitha Thomas Hans-Peter Vornlocher Arnold Ganser Felicitas Thol Michael Heuser Targeted Inhibition of the NUP98-NSD1 Fusion Oncogene in Acute Myeloid Leukemia Cancers AML NUP98-NSD1 NRASG12D siRNA liposome |
author_facet |
Sagarajit Mohanty Nidhi Jyotsana Amit Sharma Arnold Kloos Razif Gabdoulline Basem Othman Courteney K. Lai Renate Schottmann Madhvi Mandhania Johannes Schmoellerl Florian Grebien Euan Ramsay Anitha Thomas Hans-Peter Vornlocher Arnold Ganser Felicitas Thol Michael Heuser |
author_sort |
Sagarajit Mohanty |
title |
Targeted Inhibition of the NUP98-NSD1 Fusion Oncogene in Acute Myeloid Leukemia |
title_short |
Targeted Inhibition of the NUP98-NSD1 Fusion Oncogene in Acute Myeloid Leukemia |
title_full |
Targeted Inhibition of the NUP98-NSD1 Fusion Oncogene in Acute Myeloid Leukemia |
title_fullStr |
Targeted Inhibition of the NUP98-NSD1 Fusion Oncogene in Acute Myeloid Leukemia |
title_full_unstemmed |
Targeted Inhibition of the NUP98-NSD1 Fusion Oncogene in Acute Myeloid Leukemia |
title_sort |
targeted inhibition of the nup98-nsd1 fusion oncogene in acute myeloid leukemia |
publisher |
MDPI AG |
series |
Cancers |
issn |
2072-6694 |
publishDate |
2020-09-01 |
description |
NUP98-NSD1-positive acute myeloid leukemia (AML) is a poor prognostic subgroup that is frequently diagnosed in pediatric cytogenetically normal AML. NUP98-NSD1-positive AML often carries additional mutations in genes including <i>FLT3, NRAS, WT1,</i> and <i>MYC</i>. The purpose of our study was to characterize the cooperative potential of the fusion and its associated Neuroblastoma rat sarcoma (NRAS) mutation. By constitutively expressing NUP98-NSD1 and NRASG12D in a syngeneic mouse model and using a patient-derived xenograft (PDX) model from a NUP98-NSD1-positive AML patient, we evaluated the functional role of these genes and tested a novel siRNA formulation that inhibits the oncogenic driver NUP98-NSD1. NUP98-NSD1 transformed murine bone marrow (BM) cells in vitro and induced AML in vivo. While NRASG12D expression was insufficient to transform cells alone, co-expression of NUP98-NSD1 and NRASG12D enhanced the leukemogenicity of NUP98-NSD1. We developed a NUP98-NSD1-targeting siRNA/lipid nanoparticle formulation that significantly prolonged the survival of the PDX mice. Our study demonstrates that mutated NRAS cooperates with NUP98-NSD1 and shows that direct targeting of the fusion can be exploited as a novel treatment strategy in NUP98-NSD1-positive AML patients. |
topic |
AML NUP98-NSD1 NRASG12D siRNA liposome |
url |
https://www.mdpi.com/2072-6694/12/10/2766 |
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