Fluorescent Gene Tagging of Transcriptionally Silent Genes in hiPSCs

Fluorescent Gene Tagging of Transcriptionally Silent Genes in hiPSCs

Summary: We describe a multistep method for endogenous tagging of transcriptionally silent genes in human induced pluripotent stem cells (hiPSCs). A monomeric EGFP (mEGFP) fusion tag and a constitutively expressed mCherry fluorescence selection cassette were delivered in tandem via homology-directed...

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Bibliographic Details
Main Authors: Brock Roberts, Melissa C. Hendershott, Joy Arakaki, Kaytlyn A. Gerbin, Haseeb Malik, Angelique Nelson, Jamie Gehring, Caroline Hookway, Susan A. Ludmann, Ruian Yang, Amanda Haupt, Tanya Grancharova, Veronica Valencia, Margaret A. Fuqua, Andrew Tucker, Susanne M. Rafelski, Ruwanthi N. Gunawardane
Format: Article
Language:English
Published: Elsevier 2019-05-01
Series:Stem Cell Reports
Online Access:http://www.sciencedirect.com/science/article/pii/S2213671119300864

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http://www.sciencedirect.com/science/article/pii/S2213671119300864

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