Activity on non-methylated DNA limits the use of endonuclease MspJI for epigenetic analyses
Cytosine methylation of DNA in mammals has been associated with both physiological and pathological changes in gene-expression. DNA treatment with methylation sensitive and/or dependent restriction enzymes, followed by PCR amplification is a widely used approach to test CpG methylation. Recently, re...
Main Authors: | , |
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Format: | Article |
Language: | English |
Published: |
Society for the Improvement of Science (SACSIS)
2018-03-01
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Series: | All Results Journals: Biol |
Subjects: | |
Online Access: | http://arjournals.com/index.php/Biol/article/view/156 |