Construction of an Artificial MicroRNA Expression Vector for Simultaneous Inhibition of Multiple Genes in Mammalian Cells

Construction of an Artificial MicroRNA Expression Vector for Simultaneous Inhibition of Multiple Genes in Mammalian Cells

Recently, artificial microRNA (amiRNA) has become a promising RNA interference (RNAi) technology. Here, we describe a flexible and reliable method for constructing both single- and multi-amiRNA expression vectors. Two universal primers, together with two specific primers carrying the encoding sequen...

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Bibliographic Details
Main Authors: Deyin Guo, Onsam Sin, Li Ma, Ping Chen, Qiong Fu, Tao Hu
Format: Article
Language:English
Published: MDPI AG 2009-05-01
Series:International Journal of Molecular Sciences
Subjects:
artificial microRNA
expression vector
multiple genes
RNA interference
Online Access:http://www.mdpi.com/1422-0067/10/5/2158/

Internet

http://www.mdpi.com/1422-0067/10/5/2158/

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