CDetection: CRISPR-Cas12b-based DNA detection with sub-attomolar sensitivity and single-base specificity
Abstract CRISPR-based nucleic acid detection methods are reported to facilitate rapid and sensitive DNA detection. However, precise DNA detection at the single-base resolution and its wide applications including high-fidelity SNP genotyping remain to be explored. Here we develop a Cas12b-mediated DN...
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doaj-19280f8e0762415ab66d2f225d7c8b982020-11-25T03:24:39ZengBMCGenome Biology1474-760X2019-07-012011710.1186/s13059-019-1742-zCDetection: CRISPR-Cas12b-based DNA detection with sub-attomolar sensitivity and single-base specificityFei Teng0Lu Guo1Tongtong Cui2Xin-Ge Wang3Kai Xu4Qingqin Gao5Qi Zhou6Wei Li7State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of SciencesState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of SciencesState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of SciencesState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of SciencesState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of SciencesState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of SciencesState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of SciencesState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of SciencesAbstract CRISPR-based nucleic acid detection methods are reported to facilitate rapid and sensitive DNA detection. However, precise DNA detection at the single-base resolution and its wide applications including high-fidelity SNP genotyping remain to be explored. Here we develop a Cas12b-mediated DNA detection (CDetection) strategy, which shows higher sensitivity on examined targets compared with the previously reported Cas12a-based detection platform. Moreover, we show that CDetection can distinguish differences at the single-base level upon combining the optimized tuned guide RNA (tgRNA). Therefore, our findings highlight the high sensitivity and accuracy of CDetection, which provides an efficient and highly practical platform for DNA detection.http://link.springer.com/article/10.1186/s13059-019-1742-z |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fei Teng Lu Guo Tongtong Cui Xin-Ge Wang Kai Xu Qingqin Gao Qi Zhou Wei Li |
spellingShingle |
Fei Teng Lu Guo Tongtong Cui Xin-Ge Wang Kai Xu Qingqin Gao Qi Zhou Wei Li CDetection: CRISPR-Cas12b-based DNA detection with sub-attomolar sensitivity and single-base specificity Genome Biology |
author_facet |
Fei Teng Lu Guo Tongtong Cui Xin-Ge Wang Kai Xu Qingqin Gao Qi Zhou Wei Li |
author_sort |
Fei Teng |
title |
CDetection: CRISPR-Cas12b-based DNA detection with sub-attomolar sensitivity and single-base specificity |
title_short |
CDetection: CRISPR-Cas12b-based DNA detection with sub-attomolar sensitivity and single-base specificity |
title_full |
CDetection: CRISPR-Cas12b-based DNA detection with sub-attomolar sensitivity and single-base specificity |
title_fullStr |
CDetection: CRISPR-Cas12b-based DNA detection with sub-attomolar sensitivity and single-base specificity |
title_full_unstemmed |
CDetection: CRISPR-Cas12b-based DNA detection with sub-attomolar sensitivity and single-base specificity |
title_sort |
cdetection: crispr-cas12b-based dna detection with sub-attomolar sensitivity and single-base specificity |
publisher |
BMC |
series |
Genome Biology |
issn |
1474-760X |
publishDate |
2019-07-01 |
description |
Abstract CRISPR-based nucleic acid detection methods are reported to facilitate rapid and sensitive DNA detection. However, precise DNA detection at the single-base resolution and its wide applications including high-fidelity SNP genotyping remain to be explored. Here we develop a Cas12b-mediated DNA detection (CDetection) strategy, which shows higher sensitivity on examined targets compared with the previously reported Cas12a-based detection platform. Moreover, we show that CDetection can distinguish differences at the single-base level upon combining the optimized tuned guide RNA (tgRNA). Therefore, our findings highlight the high sensitivity and accuracy of CDetection, which provides an efficient and highly practical platform for DNA detection. |
url |
http://link.springer.com/article/10.1186/s13059-019-1742-z |
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