Bioorthogonal Non-canonical Amino Acid Tagging Combined With Flow Cytometry for Determination of Activity in Aquatic Microorganisms

Bioorthogonal Non-canonical Amino Acid Tagging Combined With Flow Cytometry for Determination of Activity in Aquatic Microorganisms

In this study, we have combined bioorthogonal non-canonical amino acid tagging (BONCAT) and flow cytometry (FCM) analysis, and we demonstrate the applicability of the method for marine prokaryotes. Enumeration of active marine bacteria was performed by combining the DNA stain SYBR Green with detecti...

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Bibliographic Details
Main Authors: Mathilde Lindivat, Aud Larsen, Ole Kristian Hess-Erga, Gunnar Bratbak, Ingunn Alne Hoell
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-08-01
Series:Frontiers in Microbiology
Subjects:
flow cytometry
protein synthesis
bioorthogonal non-canonical amino acid tagging
marine microbes
single cell activity
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2020.01929/full

Internet

https://www.frontiersin.org/article/10.3389/fmicb.2020.01929/full

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