Nucleosome stability measured in situ by automated quantitative imaging
Abstract Current approaches have limitations in providing insight into the functional properties of particular nucleosomes in their native molecular environment. Here we describe a simple and powerful method involving elution of histones using intercalators or salt, to assess stability features depe...
Main Authors: | , , , , , , , , , , , , , , , |
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Format: | Article |
Language: | English |
Published: |
Nature Publishing Group
2017-10-01
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Series: | Scientific Reports |
Online Access: | https://doi.org/10.1038/s41598-017-12608-9 |