Analysis of N‐glycosylation in fungal l‐amino acid oxidases expressed in the methylotrophic yeast Pichia pastoris

Analysis of N‐glycosylation in fungal l‐amino acid oxidases expressed in the methylotrophic yeast Pichia pastoris

Abstract l‐amino acid oxidases (LAAOs) catalyze the oxidative deamination of l‐amino acids to corresponding α‐keto acids. Here, we describe the heterologous expression of four fungal LAAOs in Pichia pastoris. cgLAAO1 from Colletotrichum gloeosporioides and ncLAAO1 from Neurospora crassa were able to...

Full description

Bibliographic Details
Main Authors: Marc Christian Heß, Marvin Grollius, Valentin Duhay, Simon Koopmeiners, Svenja Bloess, Gabriele Fischer von Mollard
Format: Article
Language:English
Published: Wiley 2021-08-01
Series:MicrobiologyOpen
Subjects:
fungi
glycosylation
heterologous expression
l‐amino acid oxidases
Pichia pastoris
Online Access:https://doi.org/10.1002/mbo3.1224

Internet

https://doi.org/10.1002/mbo3.1224

Similar Items