Pitfalls during in silico prediction of primer specificity for eDNA surveillance
Abstract While high efficiency and cost‐effectiveness are two merits of environmental DNA (eDNA) techniques for detecting aquatic organisms, the difficulty of designing species‐specific primers can result in significant expenditure of time and money. During the in silico stage of primer development,...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-07-01
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| Series: | Ecosphere |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/ecs2.3193 |