Note on the PCR threshold standard curve
Abstract Objective The PCR threshold standard curve is based on an exponential model of the initial phase of a PCR run where template replication efficiency is constant cycle to cycle. As such it requires that a threshold is at a level of amplified template not higher than where replication efficien...
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Format: | Article |
Language: | English |
Published: |
BMC
2017-12-01
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Series: | BMC Research Notes |
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Online Access: | http://link.springer.com/article/10.1186/s13104-017-3036-4 |