Note on the PCR threshold standard curve

Abstract Objective The PCR threshold standard curve is based on an exponential model of the initial phase of a PCR run where template replication efficiency is constant cycle to cycle. As such it requires that a threshold is at a level of amplified template not higher than where replication efficien...

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Bibliographic Details
Main Author: Benedict G. Archer
Format: Article
Language:English
Published: BMC 2017-12-01
Series:BMC Research Notes
Subjects:
PCR
Online Access:http://link.springer.com/article/10.1186/s13104-017-3036-4