Immobilization of Urease on DEAE-Cellulose Strips for One Step Urea Detection
Urease isolated from pigeonpeas was immobilized on 1 cm × 6 cm diethylaminoethyl (DEAE)-cellulose strips. The optimum immobilization (55% activity) was observed at 4°C, with a protein concentration of 1 mg per strip at pH 6.5. Immobilized strips stored at 4°C showed an increased shelf-life and there...
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| Format: | Article |
| Language: | English |
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Thieme Medical and Scientific Publishers Pvt. Ltd.
2019-01-01
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| Series: | Annals of the National Academy of Medical Sciences (India) |
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| Online Access: | http://www.thieme-connect.de/DOI/DOI?10.1055/s-0039-1694076 |