Engineering the Osmotic State of Pseudomonas putida KT2440 for Efficient Cell Disruption and Downstream Processing of Poly(3-Hydroxyalkanoates)

Engineering the Osmotic State of Pseudomonas putida KT2440 for Efficient Cell Disruption and Downstream Processing of Poly(3-Hydroxyalkanoates)

In the last decade, the development of novel programmable cell lytic systems based on different inducible genetic constructs like the holin–endolysin and lysozyme appears as a promising alternative to circumvent the use of costly enzymes and mechanical disrupters for downstream processing of intrace...

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Bibliographic Details
Main Authors: Ignacio Poblete-Castro, Carla Aravena-Carrasco, Matias Orellana-Saez, Nicolás Pacheco, Alex Cabrera, José Manuel Borrero-de Acuña
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-03-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
Pseudomonas putida
cell lysis
porins
MscL
osmotic stress
poly(3-hydroxyalkanoates)
Online Access:https://www.frontiersin.org/article/10.3389/fbioe.2020.00161/full

Internet

https://www.frontiersin.org/article/10.3389/fbioe.2020.00161/full

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