Comparison of the quantitative competitive and semiquantitative RT-PCR methods for the determination of interferon-gamma mRNA levels in AIDS-free HIV-infected individuals

Comparison of the quantitative competitive and semiquantitative RT-PCR methods for the determination of interferon-gamma mRNA levels in AIDS-free HIV-infected individuals

IFN-gamma mRNA expression was evaluated in nonstimulated peripheral blood mononuclear cells (PBMC) of HIV-infected and seronegative individuals using quantitative competitive and semiquantitative RT-PCR and the sensitivity of these methods was compared. A significant correlation was found between qu...

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Bibliographic Details
Main Authors: Barabás É., Nagy K., Várkonyi V., Horváth A.
Format: Article
Language:English
Published: Associação Brasileira de Divulgação Científica 2001-01-01
Series:Brazilian Journal of Medical and Biological Research
Subjects:
quantitative competitive RT-PCR
semiquantitative RT-PCR
IFN-gamma mRNA
HIV
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001000006

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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001000006

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