Optimization of a Density Gradient Centrifugation Protocol for Isolation of Peripheral Blood Mononuclear Cells

Optimization of a Density Gradient Centrifugation Protocol for Isolation of Peripheral Blood Mononuclear Cells

Objective: Peripheral blood mononuclear cells (PBMC) are extremely important in the body’s immune response. Their isolation represents a major step in many immunological experiments. In this two phase study, we aimed to establish an optimum protocol for PBMC isolation by density-gradient centrifugat...

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Bibliographic Details
Main Authors: Şerban Georgiana Mihaela, Mănescu Ion Bogdan, Manu Doina Ramona, Dobreanu Minodora
Format: Article
Language:English
Published: Sciendo 2018-06-01
Series:Acta Medica Marisiensis
Subjects:
density gradient centrifugation
pbmc isolation
pbmc viability
Online Access:https://doi.org/10.2478/amma-2018-0011

Internet

https://doi.org/10.2478/amma-2018-0011

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